With integrated set of separation, characterization, identification and quantification systems featured with excellent robustness & reproducibility, high and ultra-sensitivity, Creative Proteomics has developed a reliable and reproducible method using highly sensitive LC-MS/MS method for the rapid identification and quantification of Ceramide-1-phosphate in cells, which can satisfy the needs of academic and industrial study in your lab.

Overview

Ceramide-1-phosphate (C1P) is a phospho-sphingolipid that is gaining increasing recognition as an important bioactive lipid molecule. The short chain C1P induced DNA synthesis in fibroblasts. Treatment with long chain C1P was shown to have a similar effect on fibroblasts. C1P also prevented programmed cell death in bone marrow-derived macrophages after withdrawal of macrophage colony-stimulating factor. Furthermore, C1P was shown to play a distinct role in membrane fusion, possibly explaining the early finding that high levels of C1P are found in synaptic vesicles . Recent biophysical studies have shown that C1P has the ability to affect biological processes by either directly binding enzymes or via membrane deformation.

Currently, a reliable and reproducible method using highly sensitive LC-MS/MS platform for the identification and quantification of C1P in cells has been established by the scientists at Creative Proteomics, which can satisfy the needs of academic and industrial study in your lab.

Advantages of Our Ceramide-1-phosphate Analysis Service

• With integrated set of separation, characterization, identification and quantification systems featured with excellent robustness & reproducibility, high and ultra-sensitivity, Creative Proteomics provides reliable, rapid and cost-effective C1P targeted lipidomics services.
• Send us your sample and receive data in 2-4 weeks.
• A detailed report will be provided at the end of the whole project, including the experiment procedure, MS/MS instrument parameter, raw data and data reports with figures, ready to be published.

List of Detectable Ceramide-1-phosphate at Creative Proteomics

The Technical Process of Our Ceramide-1-phosphate Analysis Service

The workflow of ceramide-1-phosphate analysis service

Structural details: species level
Precision: pmol & mol%
Delivery time: 2-4 weeks
Sample requirements
Cells: 1x10⁷/sample

• Cells are cultured according to your research design.
• A minimum of 3 replicate plates or wells per condition are required.
• Remove media adjacent to the sample. The cells were washed three times with PBS and counted at the last time.
• Collect, prepare and store samples on ice.

Deliverables

• Experimental procedure
• Parameters of liquid chromatography and MS
• Purity analysis report
• MS raw data files and MS data quality checks
• Hexylceramide quantitative result data
• Custom analysis report

Based on a highly stable, reproducible and highly sensitive separation, characterization, identification and quantitative analysis system, combined with LC-MS/MS, we provide reliable, fast and cost-effective C1P analysis services. If you have any questions about our C1P targeted lipidomics services, please contact us.