Maresins are a new class of anti-inflammatory and pro-lipid mediators biosynthesized from docosahexaenoic acid (DHA) by macrophages. It has a conjugated double bond system and shows strong anti-inflammatory and solubilizing activities. Maresins limit the further recruitment of PMN, inhibit the infiltration of neutrophils in the body, and stimulate the non-inflammatory recruitment of monocytes.

When macrophages encounter maresins, they increase phagocytosis and efferocytosis, resulting in the removal of microbes. Biosynthesized maresins counterregulate the proinflammatory cytokines such as IL-1β, IL-6, and TNF-α. They also regulate nuclear factor kappa B (NF-κB) gene products and increase the regulation of T cell de novo synthesis and intracellular levels of cyclic adenosine monophosphate, regenerate tissue, and play a role in antinociceptive action.

Fig1. Maresins in the resolution pathway (Tang S.; et al, 2018)

Creative Proteomics provides comprehensive maresins analysis services from lipid extraction, lipid detection and data analysis for biomedical research institutions, biotechnology and pharmaceutical companies.

Our Maresins Analysis Service

Absolute quantification: Use external standard method to quantify, and use internal standard for correction, qualitative and quantitative is more accurate.

High sensitivity: We use liquid chromatography coupled with triple quadrupole mass spectrometers for maresins analysis service (Triple Quad™ 6500 LC-MS/MS System).

Fig 2. The workflow of maresins targeted lipidomics.

Sample Requirements

Why Choose Us?

• Fast turnaround time: 1-4 weeks.
• High throughput: Only one injection can run both quantitative MRM and qualitative QTRAP scans to maximize throughput.
• One-stop analysis service: Provide comprehensive services from sample preparation, standard addition, mass spectrometry analysis, data processing, and data comparison.
• Bioinformatics analysis: we provide data processing (e.g., data alignment, feature detection), feature prioritization (e.g., principal component analysis, heat maps, ANOVA/t-test, multivariate statistical analysis, self organizing maps, volcano plots, and network activity prediction), and lipid identification services.
• Detailed report: Experimental process, MS/MS instrument parameters, quality control parameters, raw data and the final result of the comparison.

If you have any questions about our maresins analysis services, please contact us.

Reference:

1. Tang S.; et al. Maresins: specialized proresolving lipid mediators and their potential role in inflammatory-related diseases. Mediators of inflammation. 2018.