Creative Proteomics has developed a reliable and reproducible method using highly sensitive LC-MS/MS method for the rapid identification and quantification of Rhamnolipids in yeast, which can satisfy the needs of academic and industrial study in your lab.

Overview

Biosurfactants are surface-active agents that are produced by diverse microorganisms. They exhibit surface tension reducing properties, good biodegradability, and ecotoxicity. Their potential application range includes the use in bioremediation of contaminated soils, in microbial-enhanced oil recovery (MEOR), or as an active ingredient in cleaners, foods, cosmetics, and pharmaceuticals. Among the different types, rhamnolipids are one of the most extensively studied biosurfactants and offer a convincing application potential. These glycolipids are produced in complex mixtures by bacteria of the genus Pseudomonas or Burkholderia. Their amphiphilic structure consists of one or two L-rhamnose moieties (Rha) linked in a 1,2-glycosidic linkage to up to three β-hydroxy fatty acids (β-OH-FA or 3-OH-FA) with typical chain lengths ranging from eight to sixteen.

The advantages of LC-MS/MS method include relatively higher sensitivity and selectivity and short run times. With experienced scientists at Creative Proteomics, we have developed a reliable and reproducible method using highly sensitive LC-MS/MS method for the rapid identification and quantification of rhamnolipids in yeast, which can satisfy the needs of academic and industrial study in your lab.

Our Advantages

• Send us your yeast samples and recive results in 2-4 weeks.
• Creative Proteomics full-service facility provides data processing (e.g., data alignment, feature detection), feature prioritization (e.g., principal component analysis, heat maps, ANOVA/t-test, multivariate statistical analysis, self organizing maps, volcano plots, and network activity prediction), and lipid identification services.
• Creative Proteomics provides a comprehensive visual report of the results. The report will help to answer your study questions, and hopefully create a few new ones to follow.

List of Detectable Rhamnolipids at Creative Proteomics

Our Rhamnolipids Analysis Service

Fig 2. The workflow of rhamnolipids analysis service

Structural details: species level
Mode: MRM
Variants identified: >10
Precision: pmol & mol%
Delivery time: 2-4 weeks

Deliverables

• Experimental procedure
• Parameters of liquid chromatography and MS
• Purity analysis report
• MS raw data files and MS data quality checks
• Hexylceramide quantitative result data
• Custom analysis report

Based on a highly stable, reproducible and highly sensitive separation, characterization, identification and quantitative analysis system, combined with LC-MS/MS, Creative Proteomics provides reliable, fast and cost-effective rhamnolipids analysis services. If you have any questions about our rhamnolipids targeted lipidomics services, please contact us.

Reference:

1. Behrens, Beate.; et al. Characterization of rhamnolipids by liquid chromatography/mass spectrometry after solid-phase extraction. Analytical and bioanalytical chemistry. 2016, 408.10: 2505-2514.