- Mass spectrometric-based lipidomics methods in recent years have revealed considerable complexity of lipid composition within organisms and tissues providing a wealth of information related to lipid metabolism and signaling；
- Although quite sensitive, these profiling methods rely on conventional tissue extractions of total lipids which results in a loss of original cellular context of lipid metabolites. The spatial distribution of lipids in cells is certain to be as important to their functions as their changes in concentrations;
Creative Proteomics has focused on developing approaches for high resolution sampling of lipids in plant subcellular compartments. We used direct organelle mass spectrometry (DOMS) that profiles lipids at the organelle level.
Our Services
- Direct Organelle Mass Spectrometry (DOMS)
- DOMS involves sampling organelles on a microscope stage using nano‐electrospray capillary tips
- The lipids are extracted from organelles within the capillary tip by microphase extraction, and identified and quantified by direct‐infusion nanospray MS.
- Samples
Creative Proteomics has rich experience in plant subcellular compartments untargeted lipidomics, from the commonly used model plant arabidopsis to the main food crops rice, wheat and soybeans. We routinely cover a rich variety of sample types, including lipid droplets (LDs), chloroplasts, mitochondria, plasma membranes, endoplasmic reticulum.
- Process
Fig1. The protocol workflow of plant subcellular compartments untargeted lipidomics.( Horn, Patrick J.; 2012)
Application of Plant Subcellular Compartments Untargeted Lipidomics
- Stress-induced change of plant subcellular compartments lipidomics (Heat, Freeze, Drought, Salt);
- Injury-induced change of plant subcellular compartments lipidomics;
- Development and aging induced change of plant subcellular compartments lipidomics.
Our advantages
- Send us your sample and receive data in 2-4 weeks, a low requirement for samples;
- Creative Proteomics provides a comprehensive visual report of the results. The report will help to answer your study questions, and hopefully create a few new ones to follow;
- We will use our expertise to help you interpret the results and provide you with actionable recommendations on how to utilize these results in your research or business.
If you have any questions about our plant subcellular compartments untargeted lipidomics services, welcome to contact us.
Reference:
- Horn, Patrick J.; et al. Lipidomics in tissues, cells and subcellular compartments. The Plant Journal. 2012, 70.1: 69-80.